Description
The Rat Coagulation Factor III / Tissue Factor / CD142 ELISA Pair Set is for the quantitative determination of Rat Coagulation Factor III / Tissue Factor / CD142. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Rat Coagulation Factor III / Tissue Factor / CD142 coated on a 96-well plate. Standards and samples are added to the wells, and any Rat Coagulation Factor III / Tissue Factor / CD142 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Rat Coagulation Factor III / Tissue Factor / CD142 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Rat Coagulation Factor III / Tissue Factor / CD142 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm